The transfer of proteins or nucleic acids to microporous membranes is referred to as “blotting” and this term encompasses both “spotting” (manual sample deposition) and transfer from planar gels. Proteins that are resolved on sodium dodecyl sulfate polyacrylamide

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to

Western blot is a research technique that employs the use of gel electrophoresis to separate the mixture of proteins based on molecular weight. The loss of detection of protein bands after developing the film has been a great concern to the researcher.