Gel Electrophoresis Equipment - For Western Blotting
广告Protein gel chemistry options, reagents, and equipment to help you separate your protein. Get publication-quality SDS-PAGE results the first time.Western Blot Protocols - With Positive Controls
广告Include a Lane for Positive Control in Your Western Blot. Find Your Control Today! A Better Way To Do Western Blot.
- PDF files of western blotting protocol
Learn how to perform western blotting with this comprehensive guide from Abcam. Find solutions and reagents, procedures, and useful links for sample lysis, gel electrophoresis, transfer, and antibody staining. 展开
- Preparation of lysate from cell culture
- 1. Place the cell culture dish on ice and wash the cells with ice-cold PBS. 2. Aspi…
- Preparation of lysate from tissues
- 1. Dissect the tissue of interest with clean tools, on ice preferably, an… 展开
The membrane can be either nitrocellulose or PVDF. Activate PVDF with methanol for 1 min and rinse with transfer buffer before preparing the stack. The … 展开
- 1. Remove a small volume of lysate to perform a protein quantification assay. D…
- 2. Determine how much protein to load and add an equal volume 2X Laemmli sampl…
- 3. To reduce and denature your samples, boil each cell lysate in sam… 展开
进一步探索
General Protocol for Western Blotting - Bio-Rad
网页General Protocol for Western Blotting Protein separation by gel electrophoresis 1. Load equal amounts of protein (20 μg) into the wells of a mini (8.6 x 6.7 cm) or midi (13.3 x 8.7 …
- 文件大小: 96KB
- 页数: 2
网页Learn how to perform western blotting with chemiluminescent or fluorescent detection using Thermo Fisher products and protocols. Find recipes for buffers, antibodies, and …
蛋白质印迹（Western blot）实验方案| Abcam中文官网
- 目录。溶液与试剂：裂解缓冲液。溶液与试剂：电泳、转膜及封闭缓冲液。样本裂解。样本 …
- 溶液与试剂：裂解缓冲液。这些缓冲液在 4 ℃ 下可保存数周，也可分装后在 -20 ℃ 下保存长 …
- 溶液与试剂：电泳、转膜及封闭缓冲液。Laemmli 2X 缓冲液/上样缓冲液。4% SDS。10% 2 …
- 样本裂解。细胞培养裂解液的制备。将细胞培养皿放置冰上并用冰冷的 PBS 洗涤细胞。吸出 …
- 样本制备。取少量裂解液，用于蛋白质定量分析。测定每种细胞裂解液的蛋白质浓度。确定 …
General western blot protocol - Abcam
网页Learn how to run an efficient and accurate western blot experiment with this PDF guide from Abcam. It covers solutions and reagents, sample lysis, preparation, loading and …
Western Blot 实验方案和试剂配方 | Thermo Fisher Scientific - CN
网页Western blot 或蛋白质免疫印迹是一种常规蛋白分析技术。 抗体-抗原相互作用的特异性实现了复杂蛋白质混合物中的靶蛋白识别。 在这里，您可以获取常用的蛋白质电泳 …
Addgene: Western Blot
网页2022年1月24日 · Learn how to separate and detect proteins by size using Western blot technique. Follow the step-by-step instructions for lysing cells, running SDS-PAGE gel, …
Western blot protocol | abcam
网页Western blot protocol. Comprehensive WB procedure for cell culture and tissue samples with chemiluminescent and fluorescent detection. Last edited Mon 21 Feb 2022. …
Western Blotting Solutions - PVDF Blotting Membranes
广告Analyze GST-tagged Proteins with Western Blot analysis. pore size: 0.45 um available from Merck.Advanced Structure Search · World-Class Expertise · Tech service & Support
Types: Life-Science Products, Chemicals, Laboratory Supplies, Laboratory E…Gel Electrophoresis Equipment - For Western Blotting
广告Protein gel chemistry options, reagents, and equipment to help you separate your protein. Get publication-quality SDS-PAGE results the first time.Western Blot Protocols - With Positive Controls
广告Include a Lane for Positive Control in Your Western Blot. Find Your Control Today! A Better Way To Do Western Blot.
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