网页1 天前 · Western blotting is a widely used technique for the detection and analysis of proteins based on their ability to bind to specific antibodies.. It was first described by …
WB条带分析，如何解决背景黑的问题？ - 答魔科研
网页2 天之前 · 五种情况，1、一抗浓度高了；2、封闭时间短了；3、孵育完二抗之后洗脱时间短了；4、你的蛋白有没有其他亚型；5、你的一抗来源本来就有问题，买之前说明书上面杂带多么。 0 评论 收藏 举报. 2024-05-18 …
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Method for quantitative western blotting that eliminates errors …
网页5 天之前 · The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins has been in practice for four decades. In recent years, journal editors and reviewers have requested …
A Step-by-Step Guide to Performing Western Blotting Protein
网页2 天之前 · Western blotting is a powerful and widely used technique in molecular biology and protein analysis. In this comprehensive guide provided by Kendrick Labs Inc., we will …
How to perform a western wet tank transfer using the Invitrogen …
网页4 天之前 · Assemble the western transfer sandwich by placing the cathode or negative core on a flat surface. Pour 5 milliliters of transfer buffer into the core, then place a pre-soaked sponge pad on the cathode core followed by a pre-wetted filter paper. Next, use the …
Hsc70 promotes anti-tumor immunity by targeting PD-L1 for
网页2 天之前 · PD-L1 levels were detected by western blotting (E), fluorescence of PD-L1 on the surface of cell membrane was analyzed by flow cytometry (F). G , H MCF-7 cells …
锚蛋白重复序列22（ANKRD22）对人肝癌细胞的影响及其机制
网页21 小时之前 · 通过Western Blot检测ANKRD22与细胞周期蛋白、EMT相关蛋白之间的关系。 通过KEGG、ssGSEA分析进一步探究ANKRD22在肝癌细胞中的作用机制，并进行实 …
Proteomic characterization and cytotoxic potential of proteins …
网页21 小时之前 · Western blot analysis. The effect of CE crude protein and fractions P1, P2, and P3 on the protein expression of BCL2-associated X protein (BAX) and B-cell …
Bcl-2 dependent modulation of Hippo pathway in cancer cells
网页4 天之前 · Western blotting analysis were performed to analyse protein expression of upstream regulators of YAP and in relation to different level of Bcl-2 protein. The effects …
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