The western blot is extensively used in biochemistry for the qualitative detection of single proteins and protein-modifications (such as post … 展开

Two-dimensional SDS-PAGE uses the principles and techniques outlined above. 2-D SDS-PAGE, as the name suggests, involves the migration of polypeptides in 2 dimensions. For … 展开

During the western blotting, there could be several problems related to the different steps of this procedure. Those problems could originate from a … 展开

Researchers use different software to process and align image-sections for elegant presentation of western blot results. Popular tools include Sciugo, Microsoft PowerPoint, Adobe Illustrator and GIMP. 展开

The western blot method is composed of gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide, followed by an … 展开

Detection problems
There may be a weak or absent signal in the band for a number of reasons related to the amount of antibody and antigen used. This problem … 展开

Since 1980, western blot has emerged as the most used method in molecular biology for determining the presence and quantity of a certain protein. Over the years, plenty of "advanced" and "optimized" systematical methods have been developed. These … 展开