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Western Blot Protocols - With Positive Controls
广告Include a Lane for Positive Control in Your Western Blot. Find Your Control Today! A Better Way To Do Western Blot.
A 7-Step Guide to Western Blotting
- Step 1. Sample Preparation:
- Step 2. Gel Electrophoresis:
- Step 3. Transfer:
- Step 4. Antibody Probing:
- Step 5. Detection:
- Step 6. Imaging:
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- PDF files of western blot protocol step by
Learn how to perform western blotting with this comprehensive guide from Abcam. Find solutions and reagents, procedures, and useful links for sample lysis, gel electrophoresis, transfer, antibody staining and more. 展开
- Preparation of lysate from cell culture
- 1. Place the cell culture dish on ice and wash the cells with ice-cold PBS. 2. Aspirate the PBS, then add ice-cold lysi…
- Preparation of lysate from tissues
- 1. Dissect the tissue of inte… 展开
The membrane can be either nitrocellulose or PVDF. Activate PVDF with methanol for 1 min and rinse with transfer buffer before preparing the stack. The … 展开
- 1. Remove a small volume of lysate to perform a protein quantification assay. D…
- 2. Determine how much protein to load and add an equal volume 2X Laemmli sampl…
- 3. To reduce and denature your samples, boil each cell lysate in sam… 展开
进一步探索
网页Learn how to perform western blotting with chemiluminescent or fluorescent detection using Thermo Fisher products and protocols. Find recipes for buffers, antibodies, and …
Western Blot Protocols - With Positive Controls
广告Include a Lane for Positive Control in Your Western Blot. Find Your Control Today! A Better Way To Do Western Blot.
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