The western blot is extensively used in biochemistry for the qualitative detection of single proteins and … See more

The western blot method is composed of gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide, followed by an electrophoretic transfer onto a membrane (mostly … See more

Two-dimensional SDS-PAGE uses the principles and techniques outlined above. 2-D SDS-PAGE, as the name suggests, involves the migration of polypeptides in 2 dimensions. For example, in the first dimension, … See more

There may be a weak or absent signal in the band for a number of reasons related to the amount of antibody and antigen used. This problem might be resolved by using the ideal antigen and antibody concentrati… See more

During the western blotting, there could be several problems related to the different steps of this procedure. Those problems could originate from a protein analysis step such as the detection of low- or post-translationa… See more

Researchers use different software to process and align image-sections for elegant presentation of western blot results. Popular tools include Sciugo, Microsoft PowerPoint, Adobe Illustrator and GIMP. See more